Isolation and inoculation of endophytic actinomycetes in root. Most of these isolates possessed no detectable antibiotic properties, while at least seven had antibacterial and antifungal. Isolation of endophytic actinomycetes from roots and leaves of. Precursor feeding with cacetate revealed a labeling pattern consistent with tropolone moiety construction via typeii pks. In the search of novel source of actinomycetes, endophytes have been demonstrated to improve and plant pathogens and various environmental stresses. As clear from the table 2 that diverse group of actinomycetes were isolated from different plant. Traditionally, free dwelling soil actinobacteria have been the subject of. Diversity and applications of endophytic actinobacteria of plants in. Besides these roles, they are also known to produce secondary metabolites of varied therapeutic significance. The plants after surface sterilised is checked for contamination using nutrient broth according to the method described by ei shautory et al. Synthesis and application of nanoparticles from endophytic bacteria 11. Extensive secondary metabolite analysis of the endophytic streptomyces sp. The endophytic presence of some actinomycetes may play important role in plant development and health because of their role in nutrient assimilation and in secondary metabolite production.
Assessment of plant growth promoting and abiotic stress. Screening of endophytic actinomycetes from different indigenous medicinal plants mahesh prasad 1, saneev kumar 2, umesh kumar 3 and anupama 4 1r. Investigation of bioactive compounds from endophytic actinomycete submitted by asawin wanbanjob as a partial fulfillment of the requirements for the degree. Abstract the endophytic actinomycetes constitute one of the fascinating group of microorganisms associated with a wide range of plant species. Endophytic actinomycetes from spontaneous plants of algerian sahara. Lipolytic activity tween 20 agar 30c for 7 10 days zone around the colonies 2. Isolation and bioactivity of endophytic filamentous.
Five new antitrypanosomal macrolides, actinoallolides ae 15, were discovered from the cultured broth of actinoallomurus fulvus mk10036. Secondary metabolites from endophytic streptomyces. Plant growth promotion and biological control, plant growth promoting actinobacteria, 10. Isolation and characterization of endophytic streptomycete. Isolation of endophytic actinomycetes from roots and leaves.
The goal of this study is to characterize differences in rootassociated endophytic actinobacterial community composition and. Cultivationdependent characterization of endophytic. Kibh033 revealed a new class of rubrolone analogue possessing a rare benzoic acidpyridine inner salt moiety. The strains were purified by visual, microscopic and cultivation. No enzymatic test medium used incubation indicator used method of detection 1. Isolation of actinomycetes was performed according to the method described by igarashi et al. The results of present study are comparable with the previous study on endophytes in which genus streptomyces was dominant 12. Endophytic actinomycetes from tea plants camellia sinensis. Endophytic actinomycetes, which reside within the plant tissues, are reported to have several beneficial effects on the hostplants, such as, inhibition of. The initial step, in dealing with endophytic microorganisms, is the selection of a proper and promising plant for study.
Endophytic actinomycetes in bioactive compounds production and plant defense system 10. The isolation of endophytic actinomycetes from surfacesterilized tissues of 36 plant species was made using humic acidvitamin hv agar as a selection medium. Suresh 2 1department of botany, thiagarajar college, theppakulam, madurai, tamilnadu state, india. Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that various species. Diversity and bioprospecting of actinomycete endophytes from the. The endophytic actinomycetes constitute one of the fascinating group of microorganisms associated with a wide range of plant species.
Microorganisms related to plant roots are vital for plant growth and health and considered to be the second genome of the plant. Endophytic actinomycetes as emerging source for therapeutic. A novel antibiotic source, authorravi ranjan kumar and vasantba j. Endophytic microbes associated with medicinal plants are considered to be potential producers of various bioactive secondary metabolites. Species distribution and biological diversity of endophytic actinobacteria of medicinal plants are extensively influenced by ecological environment hou et al. Jadeja, journalinternational journal of current microbiology and applied sciences, year2016, volume5, pages164175. Isolation of endophytic actinomycetes from roots and leaves of maize zea mays. Isolation and identification of endophytic actinomycetes. A complete approach article pdf available in international journal of current microbiology and applied sciences 55. Surface sterilization of 70% ethanol was used for removal of epiphytes.
However, unlike the true fungi, the actinomycetes have thin hyphae 0. Sheng qin 1, guangkai bian 1, tomohiko tamura 2, yueji zhang 1, wendi zhang 1, chengliang cao 1, jihong jiang 1. However, the snakevine kennedia nigricans of the northern territory of australia has yielded at least 39 different endophytic actinomycetes 95% of them being streptomyces spp. The diversity of actinomycetes in plants and their tissue parts is a matter of debate as no consensus are derived between individual studies. Antibacterial activity of new flavonoids from streptomyces. Studies on endophytic actinomycetes i streptomyces sp. A novel source of potential acyl homoserine lactone degrading enzymes surang chankhamhaengdecha,1,2,3 suphatra hongvijit,2,3,4,5 akkaraphol srichaisupakit,1,2,3 pattra charnchai,2,3,4 and watanalai panbangred2,3,4,5 1 department of biology, faculty of science, mahidol university, rama vi road, bangkok 10400, thailand.
Endophytic actinomycetes refer to actinomycetes that reside in the inner tissues of healthy host plants. Healthy banana plantlets are made resistance against. The qqe activity of actinomycetes in rhizospheric soil and inside plant tissue was explored in order to obtain novel strains with high hsldegrading activity. In the present field experiment, we aimed to verify the efficiency of endophytic streptomyces against one of.
Screening of isolated actinomycetes for antimicrobial activity fortyseven actinomycetes strains were isolated in accordance with differences in the colony morphology. It contained mesodiaminopimelic acid in cell wall peptidoglycans. International nano letters biogenic gold nanotriangles from saccharomonospora sp. Blast search results for strain bt01 came from genbank. Isolation, abundance and phylogenetic affiliation of. In this study, 123 endophytic actinomycetes were isolated from tropical plants collected from several locations in papua new guinea and mborokua island, solomon islands. Then, the endophytes may be successfully isolated from plant materials.
Endophytic actinomycetes inhabit tissues of a wide variety of native and cultured crop plants. Pdf endophytic actinomycetes as emerging source for. To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of rhododendron were surfacesterilized for use as an isolation source. Isolation of endophytic actinomycetes from roots and leaves of maize zea mays l. Endophytic actinomycetes, which reside within the plant tissues, are reported to have several beneficial effects on the hostplants, such as, inhibition of pathogens, increase in availability of nutrients like nitrogen and phosphorus, increasing plant growth etc. Biocontrol of chocolate spot disease botrytis cinerea in. Diversity and bioprospecting of actinomycete endophytes from. As evident from the recent literature, endophytic actinomycetes are being increasingly explored for new therapeutic molecules which may effectively address the current human health. Endophytic actinomycetes associated with cinnamomum cassia. The isolate formed a pair of ovular to circular, smoothsurfaced spores on short sporophores alternately branched from aerial mycelia. College of pharmacy, agra, uttar pradesh, india 2institute of pharmacy, monad university, uttar pradesh, india 3bhagwant institute of pharmacy, muzaffarnagar, u. These isolates belonged to streptomyces n 99, streptoverticillium n 28, and streptosporangium n 2 spp. Isolation and inoculation of endophytic actinomycetes in. Endophytic bacteria as a source of bioactive compounds 9.
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. Lorneic acid analogues from an endophytic actinomycete journal. Rubrolones are tropolonoid natural products with a unique carbon skeleton. Endophytic actinomycetes were isolated from 6 different plants collected from uttarakhand, india. Endophytes of medical plants, such as actinomycetes streptomyces sp. Sep 18, 2017 endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. Among 344 rhizospheric and 2 endophytic isolates, 127 36. Actinoallolides ae, new antitrypanosomal macrolides. The algal samples were thoroughly washed with tap water, and small fragments of. A total of 144 endophytic actinomycetes were isolated from roots, stems and leaves of aegle marmelos n51, murraya koenigii n35 and syzygium cumini n58. Identification of endophytic actinomycetes was based on their morphology and. Vijay c verma 0 1 swechha anand 1 christian ulrichs 2 santosh k singh 1 0 department of microbiology, institute of medical sciences, banaras hindu university, varanasi 221005, india 1 centre of experimental medicine and surgery, institute of medical. The list of endophytic actinomycetes isolates and their isolation source are given in table 2. It was an antagonist of colletotrichum musae and fusarium oxysporum, the causative agents of anthracnose of banana and wilt of wheat, respectively.
Various types of growth media have been described by the authors for the isolation of endophytic actinobacteria such as starch casein kuster and williams 1964, starch casein nitrate scna, actinomycetes isolation, soybean williams and davies 1965, chitinvitamin b hayakawa and nonomura 1987, tap wateryeast extract twye. Endophytic actinomycetes can be isolated by mostly chemical treatment for surface sterilization followed by serial dilution. A novel antibiotic source, authorravi ranjan kumar and vasantba. Frontiers diversity and applications of endophytic. Microorganisms free fulltext fodinicola acaciae sp. Ethyl acetate extracts of the culture supernatants from starch casein broth were prepared in vacuo. Identification of bioactive metabolites from endophytic.
Diversity and bioprospecting of actinomycete endophytes. Identification of endophytic actinomycetes was based on their morphology and the. Endophytic actinomycetes, phytopathogenic fungi, biocontrol agents, antagonistic activity. As evident from the recent literature, endophytic actinomycetes are being increasingly explored for new therapeutic molecules which. The present study investigated the distribution, antimicrobial activity and genetic features of endophytic actinomycetes isolated from the medicinal plant cinnamomum cassia presl collected in hoa binh province of northern vietnam.
Attractive biocontrol agentst hitoshi kunoh in modern agriculture, application of agrochemicals is metabolites might be promising sources of novel anti still an invaluable and effective method to control plant biotics and growth regulators of other organisms. Endophytic actinomycetes, which reside within the plant tissues, are reported to have several beneficial. Isolation and characterization of endophytic actinomycetes. This study is the first report of the isolation of endophytic actinomycetes from various medicinal plants having antagonistic and plant growth promoting abilities. Also the isolated actinomycetes were screened for antibiotic production against one yeast and three bacteria. However, a systematic investigation into the abundance and diversity of cultivated actinomycetes residing in tea plants has not been performed. Actinoallolide a showed the most potent and selective in vitro anti. Endophytic actinomycetes are a promising source of novel metabolites with diverse biological activities. Of the 330 isolates recovered, 212 were from roots, 97 from leaves and 21 isolates from stems with a prevalence of 3. Biogenic gold nanotriangles from saccharomonospora sp. Microorganisms free fulltext community structures and. Actinomycetes are one of the most attractive sources of antibiotics and other biologically active substances of high commercial value. Antibacterial effect of endophytic actinomycetes from. Endophytes are organisms, often fungi and bacteria, that live between living plant cells.
A total of 1 endophytic actinomycete strains were successfully isolated from surfacesterilized banana roots. Endophytic actinomycetes were untouched area for microbiologist, but in recent years it was found to be efficient pr oducer of novel antibiotic and lead compounds to develop new medicines. Drought tolerant endophytic actinobacteria, streptomyces coelicolor de07. Actinomycete, order actinomycetales, any member of a heterogeneous group of grampositive, generally anaerobic bacteria noted for a filamentous and branching growth pattern that results, in most forms, in an extensive colony, or mycelium. While actinomycetes could be isolated from rhizospheric soil samples, only isolates were obtained from plant samples. Isolates were found to be prevalent in roots but uncommon in leaves. Source, identification, and their applications mukesh sharma, pinki dangi and meenakshi choudhary department of biotechnology, jaipur institute of biotechnology, maharaj vinayak global university, jaipur rajasthan india corresponding author a b s t r a c t production of primary and secondary metabolites int. Among these actinomycetes isolates, the number of isolates with hsl degrading activity was found in higher frequency in endophytic isolates. Screening of endophytic actinomycetes for their herbicidal. All the 66 isolates were phenotypically grouped into 11 groups based on the presence and color of aerial mycelium, pigment and the morphology of the spore chain. A novel antibiotic source article pdf available in international journal of current microbiology and applied sciences 58. Endophytic microorganisms are rich sources for drug discovery. Pdf screening of endophytic actinomycetes for their. Evaluation of antagonistic and plant growth promoting.
Pdf diverse endophytic microbes construct complex interactions with their living hosts. Isolation of endophytic actinomycetes from selected plants. Endophytic actinomycetes as potential agents to control common scab of potatoes. Historically, the actinomycetes were called the ray fungi and were thought to be related to the true fungi, such as bread molds, because they formed mats mycelia of branching filaments hyphae. When the plant is attacked by plant pathogens, the diversity and community structure of plantassociated microbes might be changed. Tea plants camellia sinensis produce arsenals of phytochemicals, which are linked to a number of medicinal and nutritional properties. Another 71 isolates 35 from azadirachta indica, 36 from emblica officinalis were procured from the department of microbiology, pau. Jan 20, 2017 the endophytic actinomycetes constitute one of the fascinating group of microorganisms associated with a wide range of plant species. Streptomyces aureofaciens cmuac was isolated from the root tissue of zingiber officinale rosc. The surface sterilized plant parts were placed on various selective medium and were incubated at 28. One possibility is offered by local medical plants. Endophytic strains belonging to this group have been isolated from a wide range of plants, including crop plants, medicinal plants, halophytes, and even some woody tree species 7 15. The structures of the actinoallolides, including absolute stereochemistry, were elucidated by a combination of spectroscopic analyses and a series of chemical derivatization studies. Insights into the functionality of endophytic actinobacteria.
Screening of endophytic actinomycetes for their functional role is a promising approach to overcome the mounting threats of drug resistance against human and plant pathogens tan and. Journal of chemical and pharmaceutical research, 2017, 910. Plant growth promotion and biological control of pythium. The present work was carried out to isolate actinomycetes from roots and leaves of a variety of maize designated piranao, widely cultivated in brazil. The culturable diversity of endophytic actinomycetes associated with tropical, native plants is essentially unexplored. Tropolone ring construction in the biosynthesis of. Diversity and applications of endophytic actinobacteria of.
Isolation of endophytic actinomycetes the endophytes was isolated from surface sterilization method was performed by following procedure 22,23. Sustainable agriculture is needing economic applications for disease control. A novel endophytic actinomycete strain gku 173t isolated from the roots of acacia mangium willd. Isolation of actinomycetes from the surfacesterilized tissues of 12 medicinal plants in hainan, china, was carried out using different media of american type culture collection atcc 172 agar, gauzes no. The mycelium in some species may break apart to form rod or coccoidshaped forms. Actinomycetes article about actinomycetes by the free. Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that. Isolation and inoculation of endophytic actinomycetes in root nodules of elaeagnus angustifolia mingsheng chen college of life science, hebei university key lab of microbial diversity research and application of hebei prov. The isolated species showed potential for controlling collar rot disease on chickpea and could be useful in integrated control against diverse soil borne plant pathogens. Endophytic actinomycetes have been reported to inhibit the growth of soil. Isolation of endophytic actinomycetes from roots and. Almost the complete 16s rdna sequence was determined for the endophytic streptomyces sp.
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